In gel filtration chromatography
A) materials are separated based on their size, the smaller ones eluting first.
B) materials are separated based on their size, the larger ones eluting first.
C) materials are separated based on their hydrophobic nature, the more hydrophobic ones eluting first.
D) materials are separated based on their hydrophobic nature, the less hydrophobic ones eluting first.

Question

Quizplus · Accepted Answer

In gel filtration chromatography, also known as size exclusion chromatography, materials are separated based on their size, with the larger molecules eluting first because they are excluded from the pores of the gel and therefore travel more directly through the column. Smaller molecules enter the pores, which delays their elution.

In Gel Filtration Chromatography