In affinity chromatography, a protein
A) which binds to the ligand will remain on the column.
B) which binds to the ligand will elute from the column.
C) which is hydrophobic will remain on the column.
D) which is hydrophilic will remain on the column.

Question

Quizplus · Accepted Answer

In affinity chromatography, proteins that have a specific binding affinity for the ligand attached to the chromatography matrix will bind to the ligand and remain on the column. Other proteins, which do not have this specific affinity, will elute from the column.

In Affinity Chromatography, a Protein