The typical order of differential centrifugation for organelles is (from slowest speed\lowest g to fastest speed\highest g ):
A) nuclei, microsomes, mitochondria & chloroplasts, cytosol, whole cells
B) whole cells, nuclei, mitochondria & chloroplasts, microsomes, cytosol
C) cytosol, microsomes, nuclei, mitochondria & chloroplasts, whole cells
D) nuclei, mitochondria & chloroplasts, whole cells, cytosol, microsomes
E) whole cells, cytosol, microsomes, nuclei, mitochondria & chloroplasts

Question

Quizplus · Accepted Answer

Differential centrifugation separates cell components by size and density at increasing speeds. The correct order starts with whole cells (largest and least dense), followed by nuclei, then mitochondria and chloroplasts (similar in size), next microsomes (smaller vesicles), and finally the cytosol (soluble fraction).

The Typical Order of Differential Centrifugation for Organelles Is (From