Elution of proteins by means of a pH gradient would work best with this type of column:
A) Gel filtration
B) Affinity chromatography
C) Cation exchange
D) Anion exchange
E) Cation or anion exchange

Question

Quizplus · Accepted Answer

Elution of proteins by means of a pH gradient works best with ion exchange chromatography, which includes both cation and anion exchange columns. This method separates proteins based on their charge, which can be manipulated by changing the pH.

Elution of Proteins by Means of a PH Gradient Would