Which of the following happens as a protein is purified?
A) the percent recovery and the fold purification both increase
B) the percent recovery and the fold purification both decrease
C) the percent recovery increases and the fold purification decreases
D) the percent recovery decreases and the fold purification increases

Question

Quizplus · Accepted Answer

During the purification process, the percent recovery typically decreases because some amount of protein is lost at each purification step. However, the fold purification increases because the protein becomes more concentrated relative to other proteins and contaminants.

Which of the Following Happens as a Protein Is Purified