Which type of column is most affected by the shape of the protein, for example, comparing spherical and cigar-shaped proteins?
A) Gel filtration
B) Affinity chromatography
C) Cation exchange
D) Anion exchange
E) Cation or anion exchange

Question

Quizplus · Accepted Answer

Gel filtration chromatography separates molecules based on their size and shape. Spherical proteins will navigate through the matrix differently than elongated, cigar-shaped proteins, affecting their elution times. This method is particularly sensitive to the physical dimensions of the proteins being separated.

Which Type of Column Is Most Affected by the Shape