Which of the activities of DNA Polymerase I is most important in removing the primer?
A) Polymerase activity.
B) Ability to nick intact double stranded DNA.
C) 5' → 3' exonuclease.
D) 3' → 5' exonuclease.
E) None of these is used for primer removal.

Question

Quizplus · Accepted Answer

The 5' → 3' exonuclease activity of DNA Polymerase I is most important for removing the RNA primer during DNA replication. This activity allows the enzyme to remove the RNA nucleotides of the primer from the 5' end and simultaneously replace them with DNA nucleotides.

Which of the Activities of DNA Polymerase I Is Most