In an in vitro translation assay radioactive amino acids can be incorporated into proteins and the proteins separated from the unincorporated amino acids (higher counts per minute indicates more protein synthesis). Given the following data what are the conclusions that you would come to?
A) Double stranded RNA is more efficient at inhibiting translation than antisense RNA
B) Antisense RNA is more efficient at inhibiting translation than double stranded RNA
C) No conclusion about the efficiency of translation inhibition can be made as the positive and negative controls did not perform appropriately
D) The experiment did not work since the antisense fibronectin did not have an effect on amino acid incorporation.

Question

In an in vitro translation assay radioactive amino acids can be incorporated into proteins and the proteins separated from the unincorporated amino acids (higher counts per minute indicates more protein synthesis). Given the following data what are the conclusions that you would come to? 
A) Double stranded RNA is more efficient at inhibiting translation than antisense RNA 
B) Antisense RNA is more efficient at inhibiting translation than double stranded RNA 
C) No conclusion about the efficiency of translation inhibition can be made as the positive and negative controls did not perform appropriately 
D) The experiment did not work since the antisense fibronectin did not have an effect on amino acid incorporation.

Quizplus · Accepted Answer

The Answer of In an in vitro translation assay radioactive amino acids can be incorporated into proteins and the proteins separated from the unincorporated amino acids (higher counts per minute indicates more protein synthesis). Given the following data what are the conclusions that you would come to? 
A) Double stranded RNA is more efficient at inhibiting translation than antisense RNA 
B) Antisense RNA is more efficient at inhibiting translation than double stranded RNA 
C) No conclusion about the efficiency of translation inhibition can be made as the positive and negative controls did not perform appropriately 
D) The experiment did not work since the antisense fibronectin did not have an effect on amino acid incorporation.

In an in Vitro Translation Assay Radioactive Amino Acids Can