With which injection did Mello and Fire observe the lowest amount of mex-3 probe staining?
A) Injected with double-stranded RNA
B) Injected with mex-3 antisense RNA
C) Uninjected

Question

Quizplus · Accepted Answer

Mello and Fire observed the lowest amount of mex-3 probe staining when the worms were injected with double-stranded RNA. This suggests that double-stranded RNA can be used to downregulate gene expression through RNA interference. Mex-3 antisense RNA would have the opposite effect, increasing the amount of mex-3 gene expression. The uninjected group would serve as a control, but would not answer the question of which injection resulted in the lowest amount of mex-3 probe staining.

With Which Injection Did Mello and Fire Observe the Lowest