Question 1

What is proteomics?

Accepted Answer

Proteomics is the large-scale study of proteomes, which are the entire set of proteins produced or modified by an organism or system, including their structures and functions.

Question 2

Where proteomics approaches are used?

Accepted Answer

G

Question 3

What is residue of protein?

Accepted Answer

A residue of a protein refers to an amino acid that is part of a polypeptide chain.

Question 4

Peptides are linear short polymers of amino acids with less than fifty residues?

Accepted Answer

Peptides are indeed short chains of amino acids, typically consisting of fewer than fifty residues, distinguishing them from longer chains known as proteins.

Question 5

What different non-radioactive isotopic labeling techniques are used in protein purification and isolation for proteomic?

Accepted Answer

All of the listed techniques (TAP, ICAT, iTRAQ, and SILAC) are non-radioactive isotopic labeling techniques used in protein purification and isolation for proteomics.

Question 6

What is the use of stable isotope labeling by amino acid in cell culture (SILAC)?

Accepted Answer

SILAC is a technique used to identify and quantify proteins, as well as to study post-translational modifications of proteins, DNA, and RNA substrates. It involves labeling cells with stable isotopes of amino acids, such as arginine and lysine, and then analyzing the labeled proteins using mass spectrometry.

Question 7

Covalent post-translational modifications occurs in a residue of proteome?

Accepted Answer

Covalent post-translational modifications involve the chemical modification of protein residues, altering their function and activity within the proteome.

Question 8

Post translational modifications of proteins,DNA and RNA substrates occurs on arginine and lysine residues

Accepted Answer

The answer of Post translational modifications of proteins,DNA and RNA...

Question 9

Phosphorylation is a chemical process in which a phosphate group is added to an organic molecules.

Accepted Answer

Phosphorylation involves the addition of a phosphate group to an organic molecule, often regulating the activity of proteins and enzymes.

Question 10

Fill in the blanks: Phosphorylation is associated with respiration and photosynthesis which occurs in _________and _____________.

Accepted Answer

The answer of Fill in the blanks: Phosphorylation is associated...

Question 11

Glycosylation is used to study multiple functions in the cell for molecular targeting and cell-cell recognition particularly with ________,__________,and ___________.

Accepted Answer

The answer of Glycosylation is used to study multiple functions...

Question 12

Phosphorylation is a metabolic & signaling processes particularly take place in which amino acid residues____________,____________,and __________ .

Accepted Answer

The answer of Phosphorylation is a metabolic & signaling processes...

Question 13

Hydroxylation reaction is used to increase H-bonding & glycosylation sites which is primarily occurs on Pro,Lys.

Accepted Answer

Hydroxylation of proline and lysine residues in proteins increases hydrogen bonding and glycosylation sites, which is important in the stabilization of collagen.

Question 14

Hydroxylation reaction causes oxidative degradation of organic compounds in air and it is not extremely important in detoxification for rapid renal excretion.

Accepted Answer

Hydroxylation is a crucial step in the detoxification process, as it increases the solubility of organic compounds, facilitating their excretion through the kidneys.

Question 15

In which chemical process,lipophilic compounds converts into water-soluble compounds for rapid renal excretion to reduce toxicity?

Accepted Answer

Hydroxylation is a process that introduces a hydroxyl group into an organic compound, increasing its solubility in water, which aids in the excretion of lipophilic compounds through the kidneys.

Question 16

Acetylation chemical process used for charge weaken interactions with DNA and which is primarily takes place in unique amino acid residue. What is that?

Accepted Answer

Acetylation commonly occurs on the amino acid lysine, which reduces the positive charge on histones, weakening their interaction with the negatively charged DNA and affecting gene expression.

Question 17

In which chemical process co-translational and post-translational modification of proteins occurs in the cell?

Accepted Answer

The answer of In which chemical process co-translational and post-translational...

Question 18

What type of chemical groups are introduced by acetylation reaction in the cell?

Accepted Answer

Acetylation reactions introduce both acetyl groups and acetoxy groups into the cell. Acetyl groups are introduced when an acetyl-CoA molecule donates its acetyl group to a substrate, while acetoxy groups are introduced when an acetyl-CoA molecule donates its acetyl group to a hydroxyl group.

Question 19

What kind of amino acid residue play an important role in methylation?

Accepted Answer

Lysine residues are commonly involved in methylation, particularly in the context of histone modification, where methyl groups are added to lysine residues to regulate gene expression.

Question 20

By which enzyme catalysis,the carboxylation reaction introduces carbon dioxide in the DNA?

Accepted Answer

Ribulose 1,5-bisphosphate carboxylase (Rubisco) is the enzyme that catalyzes the carboxylation reaction in the Calvin cycle of photosynthesis. This reaction introduces carbon dioxide into the DNA.

Quiz 15: Drug Metabolomics and Proteomics Analysis in Drug Delivery and Discovery