What is the correct order of steps in the process of cloning a Eukaryotic gene and inserting it into a plasmid?
1) isolate the spliced mRNA that produces the desired protein
2) synthesize a cDNA using reverse transcriptase
3) transform a bacteria and select for appropriate antibiotic resistance
4) insert the cDNA into a cloning vector to form recombinant DNA

Question

Quizplus · Accepted Answer

The correct order starts with isolating the spliced mRNA (1), which is then used to synthesize a complementary DNA (cDNA) strand using reverse transcriptase (2). This cDNA is then inserted into a cloning vector, such as a plasmid, to form recombinant DNA (4). Finally, the recombinant DNA is introduced into bacteria through transformation, and bacteria with the plasmid are selected using antibiotic resistance (3).

What Is the Correct Order of Steps in the Process