Suppose Out of Budget Concerns, the Researcher Included Only Five

Suppose out of budget concerns, the researcher included only five participants in each group, tested each participant twice, and used each time as separate observations. It seems likely that

A) the assumption of independence was violated.
B) the assumption of homogeneity of variance was violated.
C) the assumption of independence of covariate and treatment was violated.
D) the assumption of homogeneity of slopes was violated.
E) there is no indication of assumption violation.