Which of the following steps comes first in next-generation sequencing?
A) Amplification of the DNA sample by polymerase chain reaction (PCR).
B) Fragmentation of the DNA sample into smaller pieces.
C) Addition of adapters to the ends of the DNA sample to allow amplification by PCR.
D) Separation of the DNA sample by electrophoresis to ensure uniform lengths.

Question

Quizplus · Accepted Answer

Fragmentation of the DNA sample into smaller pieces is usually the first step in next-generation sequencing. This can be done by physically shearing the DNA, or by using enzymes to cut it into smaller fragments. This step is necessary to create a library of DNA fragments that can be sequenced by the platform.

Which of the Following Steps Comes First in Next-Generation Sequencing