Which methods can accurately quantify the amount of mRNA expressed in a given cell or tissue sample?
A) Immunoprecipitation and mass spectrometry
B) DNA microarray and RNA-seq
C) Next-generation sequencing and tandem mass spectrometry
D) RNAi and CRISPR/Cas

Question

Quizplus · Accepted Answer

DNA microarray and RNA-seq are both methods used to quantify gene expression by measuring the amount of mRNA in a sample. DNA microarrays use immobilized probes to detect specific mRNA sequences, while RNA-seq directly sequences the entire mRNA population in a sample. These methods are widely used and provide accurate, quantitative measurements of gene expression. Immunoprecipitation and mass spectrometry, tandem mass spectrometry, RNAi, and CRISPR/Cas are all useful techniques for studying gene function, but do not specifically quantify mRNA expression levels.

Which Methods Can Accurately Quantify the Amount of MRNA Expressed