Which method for investigating protein-protein interactions uses gene expression as the detection component?
A) Yeast two-hybrid
B) Immunoprecipitation
C) Mass spectrometry
D) Tandem mass spectrometry

Question

Quizplus · Accepted Answer

The yeast two-hybrid system uses gene expression as the readout for protein-protein interactions. It relies on the ability of a transcriptional activator to bring together two fusion proteins that interact, leading to activation of a reporter gene. This system allows for identification of both direct and indirect protein interactions. Immunoprecipitation and mass spectrometry/Tandem mass spectrometry can be used to confirm protein-protein interactions that have been identified through other methods, but do not rely on gene expression as the detection component.

Which Method for Investigating Protein-Protein Interactions Uses Gene Expression as the Detection