Real time PCR can quantify DNA because …..
A) the amount of fluorescent light produced can be quantified using a spectrophotometer
B) the absence of fluorescent light produced indicates the amount of DNA amplified
C) the amount of fluorescent light produced is inversely proportional to the amount of amplified template DNA
D) the amount of fluorescent light produced is directly proportional to the amount of amplified template DNA
E) all of the above

Question

Quizplus · Accepted Answer

Real time PCR relies on a fluorescent dye that binds to double-stranded DNA as it is being amplified. As the amount of DNA increases, more fluorescent dye is bound and emitted light can be quantified in real-time. Therefore, the amount of fluorescent light produced is directly proportional to the amount of amplified template DNA.

Real Time PCR Can Quantify DNA Because …