Real time PCR produces light because of the action of the…
A) DNA polymerase exonuclease activity on a probe that contains a reporter dye and quencher
B) RNA polymerase exonuclease activity on a probe that contains a reporter dye and quencher
C) DNA polymerase exonuclease activity on a probe that contains Chelex and chloroform
D) DNA polymerase exonuclease activity on a probe that contains only adenine and guanine in the DNA template
E) RNA polymerase exonuclease activity on a probe that contains only uracil

Question

Quizplus · Accepted Answer

Real time PCR produces light because of the action of the DNA polymerase exonuclease activity on a probe that contains a reporter dye and quencher. The probe hybridizes to the target DNA and is cleaved during amplification by the 5' to 3' exonuclease activity of Taq polymerase, which separates the reporter and quencher, resulting in the release of fluorescent signal.

Real Time PCR Produces Light Because of the Action of The…