Function of the internal positive control (IPC) is to detect…
A) the presence of enzymes
B) the presence of RNA
C) the presence of primers
D) the presences of inhibitors
E) all of the above

Question

Quizplus · Accepted Answer

The function of the internal positive control (IPC) is to detect the presence of inhibitors that may be present in the sample being tested. This helps to ensure that a negative result is not due to inhibition of the amplification reaction rather than the absence of the target nucleic acid. The IPC is typically a non-target nucleic acid sequence that is added to the reaction mix along with the primers and probes for the target sequence. If the IPC fails to amplify, it indicates the presence of inhibition in the sample. The IPC does not detect enzymes or primers, and is not specific for RNA.

Function of the Internal Positive Control (IPC) Is to Detect…