Single-stranded DNA having an absorbance of 1.0 has a DNA concentration of…
A) 13 ug DNA/mL
B) 23 ug DNA/mL
C) 33 ug DNA/mL
D) 43 ug DNA/mL
E) 53 ug DNA/mL

Question

Quizplus · Accepted Answer

Single-stranded DNA has a specific molar extinction coefficient of 33 ug/mL. Therefore, a DNA concentration of 1.0 corresponds to an absorbance of 1.0/33 = 0.0303. To find the DNA concentration, we can use the formula:
Absorbance = (extinction coefficient) * (path length) * (concentration)
1.0 = 33 * 1 * (concentration)
Concentration = 1.0/33 = 0.0303 ug/mL
Multiplying by 1000 to convert to ug/mL, we get a DNA concentration of 30.3 ug/mL, which is closest to option C (33 ug/mL).

Single-Stranded DNA Having an Absorbance of 1