To identify genes in the sev pathway, sev mutants were further mutagenized and the phenotype examined. Mutations that alleviate the sev phenotype are called suppressor mutations and mutations that exacerbate the sev phenotype are called synthetic enhancer mutations.

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Mutations that alleviate a phenotype are called suppressor mutations and mutations that exacerbate a phenotype are called synthetic enhancer mutations. Secondary mutations that have an impact on the effect of the first mutation are usually in genes in the same or related pathways.

To Identify Genes in the Sev Pathway, Sev Mutants Were