Question 1

Which of the following knives is recommended for Celloidin embedded tissue blocks on a sliding microtome?
A)Biconcave knife
B)Plane-Concave knife
C)Plane-wedge knife
D)None of the above

Accepted Answer

Plane-Concave knife
The knife that is recommended for Celloidin embedded tissue blocks on a sliding microtome is the Plane-Concave knife. The less concave sides of this knife are used for cutting Celloidin tissue blocks. The other choices are not recommended for celloidin tissue blocks but rather recommended on the paraffin and frozen tissue blocks.

Question 2

This type of knife measures usually about 120 mm in length with both sides concave and it is recommended for cutting paraffin embedded sections on a rotary microtome.
A)Biconcave knife
B)Plane-Concave knife
C)Plane-wedge knife
D)None of the above

Accepted Answer

Biconcave knife
The type of knife that measures about 120 mm in length with both sides concave and is recommended for cutting paraffin embedded sections on a rotary microtome is the biconcave knife. The other choices do not fit the description of a biconcave knife.

Question 3

Which of the following knives is recommended for frozen sections or for cutting extremely hard and tough specimens?
A)Biconcave knife
B)Plane-Concave knife
C)Plane-wedge knife
D)None of the above

Accepted Answer

Plane-wedge knife
The plane-wedge knife is recommended for frozen sections or for cutting extremely hard and tough specimens. The plane-wedge knife has both sides straight that can cut frozen sections and extremely hard specimens. This knife is best combined with either using a sledge type or sliding microtome. All the other choices do not have this capability.

Question 4

This involves the removal of gross nicks on the knife edge to remove blemishes and grinding the cutting edge of the knife on a stone to acquire an even edge. What do you call this procedure?
A)Stropping
B)Honing
C)Sharpening
D)Cutting

Accepted Answer

Honing
Honing is a process that involves the removal of gross nicks on the knife edge to remove blemishes and grinding the cutting edge of the knife on a stone to acquire an even edge. This is done with the use of a hone. Other options do not accurately reflect the description of honing.

Question 5

What do you call the process that involves the removal of the burr or irregularities that are formed during honing?
A)Stropping
B)Honing
C)Sharpening
D)Cutting

Accepted Answer

Stropping
Stropping is the process that involves the removal of the burr or irregularities that is formed during honing. Stropping is the process by which the burr formed during honing is removed and the cutting edge of the knife is polished. This is for the purpose of polishing and sharpening the cutting edge of the knife. All the other choices do not fit the definition of the stropping process.

Question 6

Which of the following sharpening stones or hones gives a more polishing effect?
A)Belgium Yellow stone
B)Arkansas stone
C)Fine Carborundum stone
D)Niether A or B

Accepted Answer

Arkansas stone
The Arkansas stone is the sharpening stone or hone that gives a more polishing effect. Arkansas stones are traditional sharpening stones that are known for their hardness and fineness. Other options do not have this property.

Question 7

This type of hone is much coarser and is used only for badly nicked knives?
A)Belgium Yellow stone
B)Arkansas stone
C)Fine Carborundum stone
D)Either B or C

Accepted Answer

Fine Carborundum stone
The type of hone that is much coarser and is used only for badly nicked knives is the Fine Carborundum stone. The other choices are finer and are much recommended for polishing.

Question 8

Bryan must take note that one of the following is true about the precautions during honing.
A)The hone should be of a certain length at 8 cm by 3 cm to allow the entire knife edge to be sharpened in one stroke.
B)The pressure exerted on the knife should be kept at a steady and gentle manner to avoid it from rocking.
C)The hone shall always be lubricated with cold, soapy water before its usage.
D)The fluid used for washing must be flowing in a slow motion so that chips of metal are effectively removed.

Accepted Answer

The pressure exerted on the knife should be kept at a steady and gentle manner to avoid it from rocking.
One precaution to take during honing is to make sure that the knife should be kept at a steady and gentle manner to avoid it from rocking. Second, the hone should be of a certain length at 8 inches by 3 inches to allow the entire knife edge to be sharpened in one stroke. Third, the hone shall always be lubricated with warm, soapy water before its usage. The fluid used for washing must be flowing fast enough so that chips of metal are effectively removed.

Question 9

Chino understands that the following are precautions to observe during stropping, except:
A)Vegetable oil should be applied on the surface of the strop.
B)Pressure applied during the initial strokes of stropping should be relatively light and gentle.
C)Stopping in a quick and fast manner should be avoided.
D)Mineral oil is never used since it damages the leather.

Accepted Answer

Vegetable oil should be applied on the surface of the strop.
All of the following choices are correct about the precautions to observe during stropping, except for using vegetable oil on the surface of the strop. It is true that vegetable oil is used rather than mineral oils to avoid damaging the leather. However, vegetable oil should be applied in the backside of the strop and not on the surface of the strop.

Question 10

Don recalls that the following items are also needed during microtomy, aside from:
A)Waterbath
B)Clean slides
C)Permanent marker
D)Drying oven

Accepted Answer

Permanent marker
Aside from the microtome and microtomy knife, the histotechnician should know that other essential items needed for microtomy or sectioning are: waterbath, drying oven, forceps, clean slides, ice tray, and a pencil, not a permanent marker. Thus, choice C is the exception and is the correct answer.

Quiz 3: Microtomy

Which of the following knives is recommended for Celloidin embedded tissue blocks on a sliding microtome?

This type of knife measures usually about 120 mm in length with both sides concave and it is recommended for cutting paraffin embedded sections on a rotary microtome.

Which of the following knives is recommended for frozen sections or for cutting extremely hard and tough specimens?

This involves the removal of gross nicks on the knife edge to remove blemishes and grinding the cutting edge of the knife on a stone to acquire an even edge. What do you call this procedure?

What do you call the process that involves the removal of the burr or irregularities that are formed during honing?

Which of the following sharpening stones or hones gives a more polishing effect?

This type of hone is much coarser and is used only for badly nicked knives?

Bryan must take note that one of the following is true about the precautions during honing.

Chino understands that the following are precautions to observe during stropping, except:

Don recalls that the following items are also needed during microtomy, aside from: