Question 1

Which of the following stains is a coal tar dye?
A)Orcein
B)Saffron
C)Hematoxylin
D)Aniline dye

Accepted Answer

Aniline dye
The following stains are natural dyes except for Aniline dye. The Aniline dye is derived from the hydrocarbon, Benzene. These derived substances are known as the coal tar dyes.

Question 2

This is a natural dye derived through the extraction from the heartwood of a Mexican tree.
A)Orcein
B)Saffron
C)Hematoxylin
D)Cochineal dye

Accepted Answer

Hematoxylin
Hematoxylin is a natural dye derived through the extraction from the heartwood of a Mexican tree. The active coloring agent in this dye is hematin, which is formed by the oxidation of hematoxylin. All the other options are not extracted from the heartwood of a Mexican tree.

Question 3

What is the use of a mordant?
A)To form a bridge between the stain used and the tissue to be stained.
B)To decolorize the stained tissue.
C)To act as a counterstain that will stain the tissue with pinkish to red and the background as blue.
D)To combine aqueous solutions of acid and basic dyes.

Accepted Answer

To form a bridge between the stain used and the tissue to be stained.
The use of a mordant is to form a bridge between the stain used and the tissue to be stained. This will allow the staining reaction to occur. Without the use of Mordants, no staining could possibly take place. All the other options do not define the function of a mordant.

Question 4

What stain is used for the study of spermatogenesis?
A)Alum hematoxylin
B)Copper hematoxylin
C)Iron hematoxylin
D)Ripened hematoxylin

Accepted Answer

Copper hematoxylin
The stain used for the study of spermatogenesis is Copper hematoxylin. The other choices are not used to stain sperm for the study of spermatogenesis.

Question 5

Weigert's stain is an example of what stain?
A)Copper hematoxylin
B)Iron hematoxylin
C)Alum hematoxylin
D)Ripened hematoxylin

Accepted Answer

iron hematoxylin
Weigert's stain is an example of an iron hematoxylin compound using Iron Chloride. It is used only for differential or regressive staining. The other types of hematoxylin stains do not classify Weigert's stain under it.

Question 6

This is a fluorescent red dye commonly used as a background stain particularly in chromate and picric acid fixed tissues and in acid decalcified materials?
A)Hematoxylin
B)Eosin
C)Methylene blue
D)Iodine

Accepted Answer

Eosin
A fluorescent red dye commonly used as a background stain particularly in chromate and picric acid fixed tissues and in acid decalcified materials is Eosin. This stain gives a pleasing and colorful contrast to nuclear stains. All the other choices do not fit the description of eosin.

Question 7

Eosin is a red acid dye available in three forms. What form of eosin is most commonly used as a stain for highlighting cytoplasmic material in the samples?
A)Eosin Y
B)Eosin B
C)Eosin S
D)None of the Above

Accepted Answer

Eosin Y
Eosin is a red acid dye available in three forms. The form of eosin most commonly used as a stain for highlighting cytoplasmic material in samples is Eosin Y. It is readily soluble in water, less in alcohol, available in both aqueous and alcoholic solutions, showing green yellow fluorescence especially in alcoholic medium. All the other options are not commonly used to highlight cytoplasmic material in the samples.

Question 8

This natural dye is an excellent stain for elastic fibers and is especially recommended in dermatological studies?
A)Toluidine blue
B)Neutral red
C)Carmine
D)Orcein

Accepted Answer

Orcein
The natural dye that is an excellent stain for elastic fibers and is especially recommended in dermatological studies is Orcein. This is due to its ability to demonstrate the finest and most delicate fibers in the skin. Other options stated above are not able to highlight the delicate structures of the skin, thus they are not recommended for use in dermatological studies.

Question 9

Histological staining is the process of staining tissue constituents by direct interaction with a dye or staining solution. Listed below are classifications of histological staining, except:
A)Periodic Acid Schiff stain
B)Micro-anatomic stains
C)Bacterial stains
D)Neurologic stains

Accepted Answer

Periodic Acid Schiff stain
Histological staining is the process of staining tissue constituents by direct interaction with a dye or staining solution. All of the following are classifications of histological staining except for the Periodic Acid Schiff stain. This Periodic Acid Schiff stain is a histochemical stain that is use to stain carbohydrates.

Question 10

Natural dyes are those obtained from plants and animals. Which of the following dyes does not come from these sources?
A)Hematoxylin
B)Orcein
C)Saffron
D)Aniline

Accepted Answer

Aniline
Natural dyes are those obtained from plants and animals. Of the following dyes that do not come from these natural sources is Aniline. Synthetic dyes such as aniline are derived from the hydrocarbon benzene.

Question 11

Synthetic dyes are sometimes known as:
A)Cochineal dyes
B)Logwood dyes
C)Coal Tar dyes
D)Natural dyes

Accepted Answer

Coal Tar dyes
Synthetic dyes are sometimes known as Coal Tar dyes. They were originally manufactured from substances that have been taken from coal tar. Other dyes in the choices are natural types of dyes.

Question 12

What do you call this method of staining that uses another agent or Mordant to make the staining reaction possible?
A)Direct staining
B)Regressive staining
C)Indirect Staining
D)Progressive staining

Accepted Answer

Indirect staining
This method of staining that uses another agent or Mordant to make staining reaction possible is called Indirect staining. The action of the dye is intensified by adding another agent which serves as a link or bridge between the tissue and the dye. Other choices do not accurately describe indirect staining.

Question 13

In the indirect staining method, this non-mordant substance is not essential to the chemical union of tissue and dye and does not participate but only accelerates or hastens the speed of the staining reaction.
A)Accentuator
B)Dye
C)Mordant
D)Accelerator

Accepted Answer

Accentuator
In the indirect staining method, the non-mordant substance that is not essential to the chemical union of tissue and dye and does not participate but only accelerates or hastens the speed of staining reaction is the Accentuator. This substance increases the staining power and selectivity of the dye to accelerate the speed of the staining reaction.

Question 14

Name the substance that intensifies the action of the dye or stain in cell or tissue preparations.
A)Mordant
B)Accentuator
C)Accelerator
D)Dye

Accepted Answer

Mordant
The substance that intensifies the action of the dye or stain in cell or tissue preparations is the Mordant. It serves as a link or bridge between the tissue and the dye to make the staining reaction possible. Other options do not accurately fit the description of the question stated.

Question 15

Which among the following is a group of a metachromatic dye?
A)Thizine
B)Triphenylmethane
C)Both A and B
D)Neither A nor B

Accepted Answer

Both A and B
Metachromatic dyes are basic dyes belonging to Thizine and Triphenylmethane groups. All metachromatic dyes are cations or basic, whose peculiar staining property depends upon their tendency to polymerize. Therefore, both A and B are correct.

Question 16

What staining method involves the technique of decolorization?
A)Progressive staining
B)Regressive staining
C)Direct staining
D)Indirect staining

Accepted Answer

Regressive staining
The method of staining that involves the technique of decolorization is Regressive staining. In this technique the tissue is first over stained to obliterate the cellular details and the excess stain is removed or decolorized from unwanted parts of the tissue until the desired intensity of color is obtained. All the other options do not involve the technique of decolorization.

Question 17

In the regressive method of staining, this substance acts as a differentiator for both basic and acidic dyes?
A)Alcohol
B)Acetone
C)Xylene
D)Water

Accepted Answer

Alcohol
In the regressive staining method, the substance that acts as a differentiator for both basic and acidic dyes is alcohol. It dissolves out the excess dye in the tissue. Other choices do not act as a differentiator for both basic and acidic dyes.

Question 18

In what method of staining does the dye taken up by the tissue do not become washed or decolorized?
A)Regressive staining
B)Direct staining
C)Progressive staining
D)Metachromatic staining

Accepted Answer

Progressive staining
The method of staining wherein the dye taken up by the tissue do not become washed or decolorized is Progressive staining. In this process, the tissue elements are stained in a definite sequence and the staining solution is applied for specific periods of time. Once the dye is taken up by the tissue, it is not washed or decolorized. The differentiation of tissue detail relies solely on the selective affinity of the dye for different cellular elements. Other methods of staining in the choices do not fit the description in the question.

Question 19

All of the following are metachromatic dyes, except:
A)Safranin
B)Toluidine blue
C)Bismarck brown
D)Eosin Y

Accepted Answer

Eosin Y
All of the following are metachromatic dyes except for Eosin Y. Metachromatic dyes are basic dyes belonging to the thiazine and triphenylmethane group. Examples of such dye are safranin, toluidine blue and bismarck brown. Eosin Y is a cytoplasmic stain commonly used for counterstaining.

Question 20

Trypan blue vitally stains which specific cell structure?
A)Cytoplasm
B)Mitichondria
C)Nucleus
D)Reticulo-Endothelial System

Accepted Answer

Reticulo-Endothelial System
Trypan blue vitally stains the Reticulo-Endothelial System. Vital staining is the selective staining of living cell constituents demonstrating cytoplasmic structures by phagocytosis of the dye particle. Other specific cell structures stated in the choices are not vitally stained by trypan blue.

Quiz 5: Staining

Which of the following stains is a coal tar dye?

This is a natural dye derived through the extraction from the heartwood of a Mexican tree.

What is the use of a mordant?

What stain is used for the study of spermatogenesis?

Weigert's stain is an example of what stain?

This is a fluorescent red dye commonly used as a background stain particularly in chromate and picric acid fixed tissues and in acid decalcified materials?

Eosin is a red acid dye available in three forms. What form of eosin is most commonly used as a stain for highlighting cytoplasmic material in the samples?

This natural dye is an excellent stain for elastic fibers and is especially recommended in dermatological studies?

Histological staining is the process of staining tissue constituents by direct interaction with a dye or staining solution. Listed below are classifications of histological staining, except:

Natural dyes are those obtained from plants and animals. Which of the following dyes does not come from these sources?

Synthetic dyes are sometimes known as:

What do you call this method of staining that uses another agent or Mordant to make the staining reaction possible?

In the indirect staining method, this non-mordant substance is not essential to the chemical union of tissue and dye and does not participate but only accelerates or hastens the speed of the staining reaction.

Name the substance that intensifies the action of the dye or stain in cell or tissue preparations.

Which among the following is a group of a metachromatic dye?

What staining method involves the technique of decolorization?

In the regressive method of staining, this substance acts as a differentiator for both basic and acidic dyes?

In what method of staining does the dye taken up by the tissue do not become washed or decolorized?

All of the following are metachromatic dyes, except:

Trypan blue vitally stains which specific cell structure?