Question 1

In Counterstaining, all will color the cytoplasm red, except:
A)Eosin Y
B)Neutral Red
C)Eosin B
D)Phloxine B

Accepted Answer

Neutral Red
In counterstaining, all will stain the cytoplasm red except for Neutral Red. Counterstaining is the application of a different color or stain to provide contrast and background to the staining of structural components to be demonstrated. Examples of such stains are Eosin Y, Eosin B and Phloxine B that will stain the cytoplasm red. Neutral red will color the nucleus red.

Question 2

What part of a living cell is considered to be resistant to vital stains?
A)Nucleus
B)Mitochondria
C)RES
D)None of the above

Accepted Answer

Nucleus
The part of a living cell that is considered resistant to vital stains is the Nucleus. The nucleus is resistant to vital staining and therefore is not demonstrated. Other options above are not resistant to vital stains.

Question 3

All of the following are not supravital stains, except:
A)Lithium
B)Carmine
C)India Ink
D)Thionine

Accepted Answer

Thionine
All of the following choices are not supravital stains except for Thionine. Supravital staining is used to stain living cells immediately after removal from the living body. Common dyes used include thionine. Lithium, carmine and india ink are common intravital stains.

Question 4

Eosin Y is a counterstain that colors:
A)Red
B)Yellow
C)Blue
D)Green

Accepted Answer

Red
Eosin Y is a counterstain that colors Red. It is a common counterstain that colors the cytoplasm red. Eosin Y does not produce the other colors in the options stated above.

Question 5

Most of these fixatives can be used in Routine H & E staining in Paraffin Embedded Sections, except:
A)Alcohol
B)Osmic Acid
C)Mercuric Chloride
D)Acetone

Accepted Answer

Osmic Acid
Most fixatives can be used in routine H and E staining except for Osmic Acid. The Osmic Acid solutions will inhibit hematoxylin. All the other choices can be used in routine H and E staining.

Question 6

It is an oxazine dye used as an alternative to iron hematoxylin nuclear stain?
A)Celestine blue
B)Trypan blue
C)Nile blue
D)Toluidine blue

Accepted Answer

Celestine blue
An oxazine dye used as an alternative to iron hematoxylin nuclear stain is Celestine blue. It produces a strong and precise nuclear stain that is resistant to decolorization by succeeding acid stains and solution. Celestine blue forms a strong staining lake with iron alum, acting as a mordant to bind hematoxylin. All the other choices are not used as an alternative to iron hematoxylin nuclear stain.

Question 7

What group of dyes is insoluble in water but soluble in alcohol?
A)Acid dyes
B)Basic dyes
C)Neutral dyes
D)None of the above

Accepted Answer

Neutral dyes
The group of dyes that is insoluble in water but soluble in alcohol belongs to the group of neutral dyes. They are formed by combining aqueous solutions of acid and basic dyes. They are made up of large molecular complexes. All the other choices do not fit the specific description stated in the question.

Question 8

What specific type of stain is acid mucin?
A)Progressive stain
B)Regressive stain
C)Metachromatic stain
D)None of the above

Accepted Answer

Metachromatic stain
Acid Mucin is a type of metachromatic stain. The stain produces a color that is different from that normally exhibited by a metachromatic dye. The most useful metachromatic dye for acid mucin is Azure A. Acid mucin does not belong to the other categories of stains stated in the choices.

Question 9

Of the following choices below, which one is not a type of neutral dye?
A)Romanowsky dyes
B)Giemsa stain
C)Irishman's stain
D)Methylene blue

Accepted Answer

Methylene blue
Of the following choices given above, the one that is not a neutral dye is Methylene blue. This is because methylene blue is a basic nuclear stain that is widely used in microbiology for bacterial stain.

Question 10

What is the purpose of 10 ml ethyl alcohol in Harris Hematoxylin staining solution?
A)Antibacterial
B)Antifungal
C)Fixative
D)None of the above

Accepted Answer

Antifungal
Ethyl alcohol acts as an antifungal agent in Harris Hematoxylin staining solution. The addition of this solution will help prevent the growth of molds. All the other choices are not the functions of ethyl alcohol in Harris Hematoxylin staining solution.

Question 11

This is considered as an excellent stain for acid mucopolysaccharides. What is this specific stain?
A)Aniline blue
B)Alcian blue
C)Celestine blue
D)Congo Red

Accepted Answer

Alcian blue
Alcian blue is considered as an excellent stain for acid mucopolysaccharides. This is due to its simplicity, being resistant to various counterstaining procedures and it produces a striking blue color. All the other choices are not excellent stains used for mucopolysaccharides.

Question 12

The staining done by Janus Green B to demonstrate mitochondria is an example of what type of staining method?
A)Vital staining
B)Counterstaining
C)Direct staining
D)None of the above

Accepted Answer

Vital staining
The staining done by Janus Green B to demonstrate mitochondria is an example of vital staining. In this method, living cell constituents are selectively stained to demonstrate cytoplasmic structures and pre-existing cellular components. All the other options do not accurately describe the type of staining that Janus Green B demonstrates.

Question 13

This staining method is use in Reticulocyte counting. What do you call this method?
A)Supravital staining
B)Direct staining
C)Intravital staining
D)None of the above

Accepted Answer

Supravital staining
Supravital staining is the type of staining method use in Reticulocyte counting. Supravital staining is used to stain living cells. The RNA and the organelles precipitate, thus forming a filamentous network of reticulum. All the other choices are not the methods used in reticulocyte counting.

Question 14

Which of the following statements is not true about Mayer's Hematoxylin?
A)It is an alum hematoxylin.
B)It is used as a nuclear counterstain.
C)It is used only for regressive staining.
D)It is chemically ripened with sodium iodate.

Accepted Answer

It is used only for regressive staining
Of the following statements, the statement that is not true about Mayer's hematoxylin is that it is used only for regressive staining. Mayer's hematoxylin is also useful in progressive staining, staining tissue elements in a definite sequence.

Question 15

What staining solution does not belong to the group?
A)Ehrlich's hematoxylin
B)Harris's hematoxylin
C)Cole's hematoxylin
D)Heidenhain's hematoxylin

Accepted Answer

Heidenhain's hematoxylin
The staining solution that does not belong to the group is Heidenhain's hematoxylin. Ehrlich's hematoxylin, Harris's hematoxylin, and Cole's hematoxylin are all categorized under the alum hematoxylin solutions. Heidenhain's hematoxylin is an example of an Iron hematoxylin solution.

Question 16

Iron hematoxylin is a ferric salt used to ripen hematoxylin. Which of the following statements is not correct about iron hematoxylin?
A)It is used only for regressive staining.
B)It uses acid-alcohol as a differentiating agent.
C)Tissues structures are stained blackish or grayish.
D)The two main iron hematoxylin solutions are Weigert's and Cole's hematoxylin solution.

Accepted Answer

The two main iron hematoxylin solutions are Weigert's and Cole's hematoxylin solution.
Iron hematoxylin is a ferric salt used to ripen hematoxylin. All the other choices are correct about iron hematoxylin except for the statement that the two main iron hematoxylin solutions are Weigert's and Cole's hematoxylin solution. Cole's hematoxylin is not an iron hematoxylin solution instead it is a type of alum hematoxylin solution. The two main iron hematoxylin solutions are Weigert's and Heidenhain's solution.

Question 17

Eosin is a red dye available in how many forms?
A)1
B)2
C)3
D)4

Accepted Answer

3
Eosin is a red dye available in 3 forms. These are Yellowish (Eosin Y) which is the most common, Bluish (Eosin B) and Ethyl eosin (Eosin S). These stains are used in staining connective tissues and the cytoplasm yielding different colors for each stain.

Question 18

Among the oil soluble dyes, which dye is considered to be the most sensitive in staining intracellular fats?
A)Sudan Black B
B)Sudan III
C)Sudan IV
D)None of the above

Accepted Answer

Sudan Black B
Among the oil soluble dyes, the dye that is considered to be the most sensitive in staining intracellular fats is Sudan Black B. It demonstrates and stains intracellular fats black. All the other Sudan dyes in the choices are not as sensitive as Sudan Black B in staining intracellular fats.

Question 19

This dye is an essential component of the Schiff reagent used in histochemical staining of carbohydrates?
A)Acid fuchsin
B)Basic fuchsin
C)Carmine
D)Aniline blue

Accepted Answer

Basic fuchsin
The dye that is an essential component of the Schiff reagent used in histochemical staining of carbohydrates is Basic fuchsin. It is a mixture of 3 dyes namely rosanilin, pararosanilin and magenta H. All the other options are not essential components of the Schiff reagent.

Question 20

In glycogen demonstration, what stain is widely used?
A)Methylene blue
B)Aniline blue
C)Carmine
D)Periodic Acid Schiff

Accepted Answer

Periodic Acid Schiff
In glycogen demonstration, the stain that is widely used is Periodic Acid Schiff. The reaction of Periodic Acid Schiff with or without diastase is considered a specific test for glycogen. All the other choices are not widely used in glycogen demonstration.

Quiz 5: Staining

In Counterstaining, all will color the cytoplasm red, except:

What part of a living cell is considered to be resistant to vital stains?

All of the following are not supravital stains, except:

Eosin Y is a counterstain that colors:

Most of these fixatives can be used in Routine H & E staining in Paraffin Embedded Sections, except:

It is an oxazine dye used as an alternative to iron hematoxylin nuclear stain?

What group of dyes is insoluble in water but soluble in alcohol?

What specific type of stain is acid mucin?

Of the following choices below, which one is not a type of neutral dye?

What is the purpose of 10 ml ethyl alcohol in Harris Hematoxylin staining solution?

This is considered as an excellent stain for acid mucopolysaccharides. What is this specific stain?

The staining done by Janus Green B to demonstrate mitochondria is an example of what type of staining method?

This staining method is use in Reticulocyte counting. What do you call this method?

Which of the following statements is not true about Mayer's Hematoxylin?

What staining solution does not belong to the group?

Iron hematoxylin is a ferric salt used to ripen hematoxylin. Which of the following statements is not correct about iron hematoxylin?

Eosin is a red dye available in how many forms?

Among the oil soluble dyes, which dye is considered to be the most sensitive in staining intracellular fats?

This dye is an essential component of the Schiff reagent used in histochemical staining of carbohydrates?

In glycogen demonstration, what stain is widely used?