Question 1

All of the following are standard MSB techniques for fibrin except for:
A)Martius Yellow
B)Brilliant crystal scarlet
C)Soluble blue
D)Verhoeff's

Accepted Answer

Verhoeff's
All of the following are standard MSB techniques for fibrin except for Verhoeff's. It is used to stain elastic fibers.

Question 2

This stain is useful in the detection of myelofibrosis?
A)Methyl-Green Pyronin
B)Perl's stain
C)Reticulin stain
D)Toluidine

Accepted Answer

Reticulin stain
The stain that is useful in the detection of myelofibrosis is Reticulin stain. In myelofibrosis, reticulin is greatly increased and this stain can therefore be used to detect myelofibrosis. All the other stains are not used for this purpose.

Question 3

In this method of staining myelin sheaths, the mordant and the dye are mixed in the staining solution. This method is called?
A)Weil's method
B)Luxol fast blue
C)Wegert-Pal
D)Kleiver and Barrera

Accepted Answer

Weil's method
In the method of staining myelin sheaths, the mordant and the dye are mixed in the staining solution. The method is called Weil's method. It is a good method used for frozen sections. All the other options do not fit the description stated in the question.

Question 4

All of the following are Romanowsky stains except for:
A)Giemsa
B)Leishman
C)May Grunwald
D)Krajan's

Accepted Answer

Krajan's
All of the following choices are Romanowsky stains except for Krajan's. This is a rapid method for staining elastic fibers.

Question 5

In re-staining of Old sections, the section is placed in xylene for how many minutes?
A)10 minutes
B)20 minutes
C)30 minutes
D)40 minutes

Accepted Answer

30 minutes
In re-staining of Old sections, the section is placed in xylene for 30 minutes. This is to remove the remaining balsam in the section. Then the section is brought down to water. The duration stated in the other choices are inaccurate.

Question 6

This type of dye is derived from the extracts of female bugs called as Coccus cacti, which is treated with alum to produce the dye, Carmine?
A)Orcein
B)Hematoxylin
C)Cochineal dye
D)Saffron

Accepted Answer

Cochineal dye
Cochineal dye is the type of dye that is derived from the extracts of female bugs called as Coccus cacti. When it is treated with alum, it produces the dye called Carmine. This is a classic histological dye derived from an extract of Coccus cacti.

Question 7

This type of stain is derived from certain Lichens which are normally colorless?
A)Orcein
B)Saffron
C)Cochnineal dye
D)Hematoxylin

Accepted Answer

Orcein
Orcein is the type of stain that is derived from certain Lichens, which are normally colorless. Orcein is colorless, but when treated with Ammonia and is exposed to air, it produces blue and violet colors.

Question 8

What is the possible reason why Orcein is not used as a cytological stain?
A)It is a synthetic dye.
B)It is a natural dye.
C)It has a poor staining property.
D)It is a colorless substance.

Accepted Answer

It has a poor staining property.
The possible reason why Orcein is not used as a cytological stain is due to its poor staining property. It is rather used as an indicator. Other reasons in the choices are not the reasons why Orcein is not used as a cytological stain.

Question 9

What type of stain is used extensively for neuropathological studies?
A)Orcein
B)Cochineal dye
C)Saffron
D)Hematoxylin

Accepted Answer

Cochineal dye
The type of stain that is used extensively for neuropathological studies is the Cochineal dye. It is an extensive and powerful chromatin and nuclear stain used for the demonstration of Glycogen and as well as a stain for fresh material and smear preparations.

Question 10

Which type of stain is described as by far the most valuable staining reagent used by cytologists due its powerful nuclear and chromatin staining capacity?
A)Hematoxylin
B)Cochineal dye
C)Saffron
D)Orcein

Accepted Answer

Hematoxylin
The type of stain that is described as by far the most valuable staining reagent used by cytologists due to its powerful nuclear and chromatin staining is Hematoxylin. The active coloring agent of this stain is Hematin, which is formed by the oxidation of Hematoxylin.

Question 11

When using Alum Hematoxylin for the progressive staining of tissues, what is the usual counterstain to be used?
A)Eosin
B)Congo red
C)Saffranin
D)All of the above

Accepted Answer

All of the above
When using Alum Hematoxylin for the progressive staining of tissues, the usual counterstain to be used are eosin, Congo red, and saffranin. Therefore, all the choices stated above are correct.

Question 12

What does ripening refer to in the context of Hematoxylin staining?
A)Combination of Hematoxylin to other stains
B)Oxidation of Hematoxylin
C)Use of Hematoxylin stain as a regressive stain
D)To form a bridge between other stains

Accepted Answer

Oxidation of Hematoxylin
Ripening refers to the process whereby Hematoxylin undergoes the process of oxidation. This is usually done by exposing the substance to air and sunlight. All the other choices do not accurately describe what ripening is, in the context of hematoxylin staining.

Question 13

Harris' solution is an alum hematoxylin. Which of the following is used to oxidize Harris' solution?
A)Mercuric chloride
B)Sodium iodate
C)Ferric chloride
D)Potassium iodate

Accepted Answer

Sodium iodate or potassium iodate are used to oxidize Harris' solution
For Harris' hematoxylin solution, the oxidizing agent can be either sodium or potassium iodate. Mercuric chloride was previously used to oxidize Harris' solution, but is no longer used due to its toxicity. Ferric chloride is used to oxidize Weigert's solution, an iron hematoxylin.

Question 14

What solution is added to Ehrlich's reagent for it to ripen rapidly?
A)Mercuric Chloride
B)Hydrogen Peroxide
C)Sodium Iodate
D)Potassium Permanganate

Accepted Answer

Sodium Iodate
The solution that is added to Ehrlich's reagent for its rapid ripening is Sodium Iodate. The other choices written above are all oxidizing agents that also hasten the ripening of Ehrlich's reagent. However, these are not the oxidizing agent of choice for Ehrlich's reagent.

Question 15

What is the reason why Ripened Hematoxylin is seldom used alone?
A)Due to its inherent low affinity for the tissue itself.
B)Too much color produced during staining
C)It is easily decolorized.
D)It has greater affinity with the basic dyes.

Accepted Answer

Due to its inherent low affinity for the tissue itself.
The possible reason why Ripened Hematoxylin is seldom used alone is due to its inherent low affinity for the tissue itself. Because of this, it is frequently combined with Alum, Iron, Chromium, and Copper Salts.

Question 16

These are dyes that are derived from the hydrocarbon Benzene. These are collectively known as?
A)Cochineal Dye
B)Orcein
C)Aniline Dyes
D)Hematoxylin

Accepted Answer

Aniline Dyes
The dyes that are derived from the hydrocarbon Benzene are collectively known as Aniline dyes. These are synthetic dyes that are originally manufactured from substances that have been taken from Coal Tar which were derived from the benzene. The other types of dyes in the choices are not derived from the hydrocarbon, benzene.

Question 17

What stain is routinely used for nuclear staining in Exfoliative Cytology and for staining sex chromosomes?
A)Ehrlich's Hematoxylin
B)Harris Hematoxylin
C)Cole's Hematoxylin
D)Iron Hematoxylin

Accepted Answer

Harris Hematoxylin
The stain that is routinely used for nuclear staining in Exfoliative Cytology and for staining sex chromosome is Harris Hematoxylin. The usual staining time for this is 5-20 minutes. Other stains in the choices are not routinely used for nuclear staining and for staining sex chromosomes.

Question 18

When sections are stained with simple Aqueous or Alcoholic solutions of the dye, what you call this staining process?
A)Progressive staining
B)Regressive staining
C)Direct staining
D)Indirect staining

Accepted Answer

Direct staining
When sections are stained with simple Aqueous or Alcoholic solutions of the dye, the staining process is called as direct staining. The other choices are incorrect and do not fit the description in the question.

Question 19

This is not essential to the chemical union of the tissue and the dye and it does not participate in the staining reaction. What is this substance?
A)Mordant
B)Accentuator
C)Water
D)Aniline water

Accepted Answer

Accentuator
The accentuator is not essential to the chemical union of the tissue and the dye and it does not participate in the staining reaction. The Accentuator functions to accelerate or hasten the speed of the staining reaction by increasing the staining power and selectivity of the dye. The other choices do not fit the characteristics of an accentuator.

Question 20

This is a substance that serves as a link or bridge to make the staining reaction possible when it is taken up by the tissue.
A)Accentuator
B)Mordant
C)Aniline Dye
D)Hematoxylin

Accepted Answer

Mordant
The mordant is a substance that, when taken up by the tissue, serves as a link or bridge to make the staining reaction possible. All the other choices do not fit the definition of a mordant.

Quiz 5: Staining

All of the following are standard MSB techniques for fibrin except for:

This stain is useful in the detection of myelofibrosis?

In this method of staining myelin sheaths, the mordant and the dye are mixed in the staining solution. This method is called?

All of the following are Romanowsky stains except for:

In re-staining of Old sections, the section is placed in xylene for how many minutes?

This type of dye is derived from the extracts of female bugs called as Coccus cacti, which is treated with alum to produce the dye, Carmine?

This type of stain is derived from certain Lichens which are normally colorless?

What is the possible reason why Orcein is not used as a cytological stain?

What type of stain is used extensively for neuropathological studies?

Which type of stain is described as by far the most valuable staining reagent used by cytologists due its powerful nuclear and chromatin staining capacity?

When using Alum Hematoxylin for the progressive staining of tissues, what is the usual counterstain to be used?

What does ripening refer to in the context of Hematoxylin staining?

Harris' solution is an alum hematoxylin. Which of the following is used to oxidize Harris' solution?

What solution is added to Ehrlich's reagent for it to ripen rapidly?

What is the reason why Ripened Hematoxylin is seldom used alone?

These are dyes that are derived from the hydrocarbon Benzene. These are collectively known as?

What stain is routinely used for nuclear staining in Exfoliative Cytology and for staining sex chromosomes?

When sections are stained with simple Aqueous or Alcoholic solutions of the dye, what you call this staining process?

This is not essential to the chemical union of the tissue and the dye and it does not participate in the staining reaction. What is this substance?

This is a substance that serves as a link or bridge to make the staining reaction possible when it is taken up by the tissue.