The correct order of steps in each cycle of the polymerase chain reaction are:
I. The DNA sample is heated to unwind the double helix into two single stands.
II. Primers are added to form a short segment of double-stranded DNA on each strand
III. DNA polymerase is used to add nucleotides to lengthen the DNA segment

Question

Quizplus · Accepted Answer

The correct order for a PCR cycle is: first, the DNA is heated to separate the strands (I), then primers are added to initiate replication (II), and finally, DNA polymerase extends the DNA from the primers (III).

The Correct Order of Steps in Each Cycle of the Polymerase