In size exclusion chromatography
I. molecules too large to interact with beads elute in the void volume
II. molecules of intermediate size may enter the gel pores and are
Retained to a lesser extent than the largest molecules
III. beads and buffer together are termed the "bed volume"
IV. the elution volume is that at which the targeted protein elutes

Question

Quizplus · Accepted Answer

Molecules too large to enter the pores elute in the void volume (I). The bed volume includes both beads and buffer (III). The elution volume is the volume at which the targeted protein elutes (IV).

In Size Exclusion Chromatography