In size exclusion chromatography, resins with small pore sizes will be able to resolve large molecules but not small ones. Therefore, the size of the pore must be matched to the estimated size of the protein to be purified.

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Quizplus · Accepted Answer

In size exclusion chromatography, resins with small pore sizes will actually exclude large molecules, allowing them to elute first, while smaller molecules enter the pores and are eluted later. Therefore, the pore size must indeed be matched to the size of the protein for effective separation, making the second statement true.

In Size Exclusion Chromatography, Resins with Small Pore Sizes Will