If the following mixture of proteins was applied to a size-exclusion chromatography column, what would be the order of elution?
Proteins with molecular weights: myoglobin (17.7 kDa) , hemoglobin (64.5 kDa) , lysozyme (14.3 kDa) and triose phosphate isomerase (57.4 kDa)
A) lysozyme, myoglobin, triose phosphate isomerase, hemoglobin
B) triose phosphate isomerase, hemoglobin, lysozyme, myoglobin
C) hemoglobin, myoglobin, lysozyme, triose phosphate isomerase
D) hemoglobin, triose phosphate isomerase, myoglobin, lysozyme,
E) cannot be determined
Correct Answer:
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A)
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I. molecules too large
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A) an electropositive
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