Which assay would allow you to determine whether two transcription factors with nearby binding sites in a promoter sequence bind to the sequence synergistically?

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Quizplus · Accepted Answer

EMSA (Electrophoretic Mobility Shift Assay) allows for the observation of protein-DNA interactions, including the binding of transcription factors to DNA. By comparing the mobility shifts of DNA-protein complexes with individual transcription factors to those with both factors present, one can determine if there is synergistic binding.

Which Assay Would Allow You to Determine Whether Two Transcription