The chromatographic method based on biologically related interactions by immobilizing one of a pair of interacting biomolecules onto a solid support and placing it in a column is known as:

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Affinity chromatography is a technique that separates molecules based on a highly specific interaction between an antigen and antibody, enzyme and substrate, or receptor and ligand. One of the interacting molecules is immobilized on a stationary phase, and the target molecule is selectively captured from the mixture passing through the column.

The Chromatographic Method Based on Biologically Related Interactions by Immobilizing