Given that the base peak (m/z = 112) in a mass spectrum has an initial relative abundance of 75% and the peak at m/z 125 has an initial relative abundance of 30%, what will be the relative abundance of the two peaks following normalization? (answer format: relative abundance for m/z 112, relative abundance for m/z 125)

Question

Quizplus · Accepted Answer

The most intense ion in the spectrum is known as the base peak.The base peak is assigned a relative abundance of 100%.The relative abundance of all other peaks in the spectrum are then normalized (i.e., adjusted by ratio) to the base peak.For this data, the peak at m/z 112 will be adjusted to a relative abundance of 100%.For the peak at m/z 125, the ratio will be:
100%/75% = x/30%; x = 40% (normalized relative abundance for m/z 125)

Given That the Base Peak (M/z = 112) in a Mass