Consider the separation of Hb 1, Hb 2, and Hb 3 by cation-exchange HPLC.The initial mobile phase pH causes Hb 1 to have the greatest positive charge, Hb 2 to have minimal positive charge, and Hb 3 to be neutral.Predict the order of elution from the stationary phase as the chromatographic run continues from the initial mobile phase to mobile phases with increasing concentrations of cations.(first to elute to last to elute)

Question

Quizplus · Accepted Answer

Hb 3 has no net charge and will show little if any retention by the cation-exchange
stationary phase.Hb 2 has minimal positive charge and will show a small degree of retention by the stationary phase.Hb 1, with the greatest degree of positive charge, will be retained to the greatest extent by the stationary phase.

Consider the Separation of Hb 1, Hb 2, and Hb