Isoelectric Focusing (IEF) May Be Utilized to Investigate Unresolved HPLC

Isoelectric focusing (IEF) may be utilized to investigate unresolved HPLC hemoglobin results.What advantage of this technique makes it useful in this situation?

A) IEF is a technique that is readily adaptable to automation, resulting in a quick and simple method to resolve questionable HPLC hemoglobin results.
B) IEF is a technique that does not depend on the charge of the hemoglobin fractions, providing a method that complements HPLC analysis of hemoglobin fractions.
C) IEF results in the formation of discrete sharp bands, allowing clear separation of the various hemoglobins present in the patient sample.
D) IEF provides an enhanced method of detection that may allow the technologist to see fractions not detected by HPLC.