Quantification of Hb A2 following separation by cation-exchange HPLC produced a result of 15% in an Hb AA patient.Which of the following describes a concern the technologist should have regarding this result?
A) There may be other hemoglobin variants co-eluting with Hb A2, causing the Hb A2 result to be falsely elevated.
B) The low Hb A2 result may be falsely decreased because of an integration error caused by a rising baseline.
C) Quantification of Hb A2 by HPLC is no longer recognized by CAP because of significant intralaboratory variation in Hb A2 measurements.
D) Glycation of Hb A2 may cause its concentration to vary significantly, based on the extent to which Hb A2 is glycated.
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