Hb F is the major hemoglobin fraction present in neonates and is found elevated in various hematologic conditions.Which of the following problems may be encountered in the HPLC analysis of Hb F when it is greatly elevated?
A) The measured retention time may fall outside the Hb F window, causing the peak to be labeled as "unknown."
B) Hb F will be measured as part of the background and produce no discernable chromatographic peak
C) Hb F will crystallize and block flow through the chromatographic column.
D) Hb F will break into fragments, producing multiple peaks for detection.
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