In the chromatographic analysis of variant hemoglobins, it is the retention time that determines the _________ and the absorbance that determines the _________.

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The retention time is based on the unique interaction of each hemoglobin variant and the stationary phase.Thus retention time is an indication of the hemoglobin variant identity.Based on Beer's Law, as concentration increases, absorbance will increase.Considering all peaks (hemoglobins) present, measuring the absorbance of each peak will allow the determination of the percent concentration of an individual peak (hemoglobin).

In the Chromatographic Analysis of Variant Hemoglobins, It Is the Retention