Suppose HPLC results suggest Hb S trait.Why should this be confirmed with the sickle solubility test?
A) Retention characteristics of hemoglobins vary significantly between chromatographic runs, requiring this confirmation step.
B) With chromatography, there is always the possibility of unknown hemoglobins co-eluting with the suspected hemoglobin.
C) The use of chromatographic windows has been associated with a high degree of imprecision.
D) The sickle solubility test is the only technique available to quantify Hb S.
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