Standard Hemoglobin Electrophoresis on Cellulose Acetate at PH 8

Standard hemoglobin electrophoresis on cellulose acetate at pH 8.6 is utilized in some laboratories for the evaluation of hemoglobins.In many cases, this is followed by hemoglobin electrophoresis on the same patient sample, using citrate agar at pH 6.2.What is the purpose of this second electrophoretic analysis?

A) to confirm the results of the electrophoresis in the cellulose acetate (alkaline) system
B) to aid in the identification of hemoglobins that co-migrate in the cellulose acetate (alkaline) system
C) to replace the need for chromatographic analysis
D) to give the technologist confidence that the initial results are correct, since the second technique will produce identical results as the first technique