Hb F may be acetylated in vivo.What is the effect of acetylated Hb F on the measurement of total Hb F (acetylated and non-acetylated) by HPLC?
A) The peak produced by Hb F will be much larger in the presence of the acetylated form because of the increased absorptivity of the acetyl group, resulting in an overestimation of total Hb F levels.
B) The acetylated form of Hb F cannot be detected by HPLC systems, causing the level of total Hb F to be underestimated.
C) The acetylated form of Hb F will have a different retention time from the non-acetylated form, resulting in the need to sum both forms to determine the total Hb F level.
D) The presence of the acetylated form of Hb F will produce baseline drift, causing the analysis of total Hb F to be unreliable.
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