The real-time PCR technique that utilizes two fluorophores where the emission spectrum of one fluorophore overlaps the excitation spectrum of the second fluorophore is known as the:

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The use of two fluorophores where the emission spectrum of one fluorophore overlaps with the excitation spectrum of the other is known as fluorescence resonance energy transfer (FRET).These two probes bind to the target sequence.When the two probes come in close proximity during hybridization, energy transfer will occur, resulting in fluorescence at the appropriate wavelength.

The Real-Time PCR Technique That Utilizes Two Fluorophores Where the Emission