Single-stranded conformational polymorphism analysis (SSCP) and heteroduplex analysis are two mutation scanning techniques that have which of the following in common?

Question

Quizplus · Accepted Answer

Both SSCP and heteroduplex techniques are based on the fact that the three-dimensional conformation of DNA with mutation will be different from DNA fragments without sequence variations.Electrophoretic mobility is a function of the shape of the migrating molecule.Thus if the sequence of a sample PCR product is different from a reference fragment because of a mutation, it will adopt a different three-dimensional conformation and exhibit unique electrophoretic mobility.

Single-Stranded Conformational Polymorphism Analysis (SSCP) and Heteroduplex Analysis Are Two