You are studying the replication characteristics of a cell line in culture. You find that the DNA in these cells fragments easily and does not replicate well. Analysis of components of the replication machinery and associated compounds shows that the levels of spermidine and spermine are significantly reduced compared to normal cells. A deficiency in which of the following enzymes could best explain the observations made with these cells?

Question

Quizplus · Accepted Answer

Ornithine decarboxylase is involved in the synthesis of polyamines like spermidine and spermine, which are important for DNA stabilization and replication. A deficiency in this enzyme would lead to reduced levels of these polyamines, explaining the observed DNA fragmentation and poor replication.  Spermine and spermidine are polyamines whose functions include stabilization of DNA during the processes of replication. The initial step in polyamine synthesis is catalyzed by ornithine decarboxylase (ODC), which decarboxylates the amino acid ornithine producing putrescine

You Are Studying the Replication Characteristics of a Cell Line