Which of the following is the effect of adding oligomycin and then 2,4-dinitrophenol to respiring mitochondria?
A)Electron transport speeds up and ATP synthesis stops.
B)Electron transport continues and ATP synthesis continues.
C)Electron transport stops and ATP synthesis stops.
D)Electron transport stops and ATP synthesis continues.

Question

Quizplus · Accepted Answer

Oligomycin inhibits ATP synthase, the enzyme responsible for ATP synthesis. By adding oligomycin, ATP synthesis would stop, but electron transport would continue. However, by adding 2,4-dinitrophenol, a mitochondrial uncoupler, the proton gradient that drives ATP synthesis is disrupted, allowing electron transport to speed up but without any ATP synthesis occurring. Therefore, the net effect is that electron transport speeds up and ATP synthesis stops.

Which of the Following Is the Effect of Adding Oligomycin