How is radioactivity incorporated into newly synthesized DNA using the chain termination protocol for sequencing DNA? A)One of the dNTPs is labelled with ³²P. B)The primer strand is labelled with ³²P. C)The 5'-end is labelled with ³²P. D)The dideoxynucleotide is labelled with ³²P.

Question

Quizplus · Accepted Answer

In the chain termination protocol, fluorescently labelled dideoxynucleotides are incorporated into the newly synthesized DNA. However, in the radioactive version of this protocol, one of the normal dNTPs (not the dideoxynucleotide) is labelled with a radioactive isotope, typically 32P. This allows for detection of the newly synthesized DNA via autoradiography. Therefore, choice A is the best option as it describes how radioactivity is incorporated into the newly synthesized DNA during sequencing.

How Is Radioactivity Incorporated into Newly Synthesized DNA Using the Chain