Which of the following pairs of nucleotides,present in high concentrations,would result in a higher DNA melting temperature?
A)A:T
B)A:U
C)G:C
D)I:G

Question

Quizplus · Accepted Answer

The melting temperature of DNA depends on the strength of the hydrogen bonds between the complementary nucleotides. The combination of G:C base pairs has three hydrogen bonds, whereas A:T base pairs have only two hydrogen bonds. Therefore, the presence of G:C base pairs in high concentrations results in a higher melting temperature. A:U is not present in DNA, and I:G is an incorrect nucleotide pairing.

Which of the Following Pairs of Nucleotides,present in High Concentrations,would