The UV absorption decrease associated with ssDNAs reannealing can be plotted on a c₀t curve.Which of the following does this graph indicate?
A)that more complex DNAs take longer to renature
B)that more double-stranded RNAs take longer to renature
C)that more simple DNAs take longer to renature
D)that more single-stranded RNAs take longer to renature

Question

Quizplus · Accepted Answer

The c₀t curve, which plots the reannealing kinetics of single-stranded DNA (ssDNA) as a function of concentration and time, indicates that more complex DNAs (with longer sequences or more unique sequences) take longer to renature. This is because complex DNAs have more potential pairing sites and thus require more time for all complementary strands to find each other and hybridize.

The UV Absorption Decrease Associated with SsDNAs Reannealing Can Be