How do urea and formamide denature dsDNA?
A)by intercalating between base pairs and disrupting van der Waals interactions
B)by forming ionic bonds with the backbone phosphates
C)by competing effectively with the H-bonding between the base pairs
D)by changing the pH to cause hydrolysis

Question

Quizplus · Accepted Answer

Urea and formamide disrupt the H-bonding between the base pairs by competing effectively with hydrogen bonding. This leads to denaturation of dsDNA.

How Do Urea and Formamide Denature DsDNA