Which of the following is the mechanism of action of 5-fluorouracil?
A)competitive inhibitor of dihydrofolate reductase
B)competitive inhibitor of ribonucleotide reductase
C)suicide substrate for thymidylate synthase
D)non-competitive inhibitor of thioredoxin reductase

Question

Quizplus · Accepted Answer

5-fluorouracil acts as a suicide substrate for thymidylate synthase, an enzyme involved in the synthesis of DNA. The drug is metabolized to 5-fluoro-2'-deoxyuridine monophosphate (FdUMP), which binds covalently to the enzyme and inhibits its activity, leading to decreased production of thymidine and ultimately, DNA synthesis. This results in cell cycle arrest and ultimately apoptotic cell death.

Which of the Following Is the Mechanism of Action of 5-Fluorouracil