Which of the following is NOT a part of the procedure associated with the Southern blotting (hybridization)technique?
A)hybridization with radioactive probe
B)agarose gel electrophoresis and visualize bands
C)transfer (blot)to nitrocellulose filter
D)digestion of DNA with DNA ligase

Question

Quizplus · Accepted Answer

DNA ligase is not involved in the Southern blotting technique. The steps involved in Southern blotting include agarose gel electrophoresis of DNA fragments, transfer of the fragments to a nitrocellulose filter or nylon membrane, hybridization of the membrane with a labeled DNA probe, and visualization of the bands using autoradiography or other detection methods.

Which of the Following Is NOT a Part of the Procedure