Which of the following is NOT a part of the process associated with colony hybridization experiments?
A)A replica of the bacterial colonies is obtained on an absorbent disc.
B)Autoradiography of the disc reveals probe-complementary DNA.
C)Host bacteria with plasmid are plated and allowed to grow without media for days.
D)The disc is treated with alkali.

Question

Quizplus · Accepted Answer

The process of colony hybridization experiments includes obtaining a replica of the bacterial colonies on an absorbent disc, treating it with alkali, blotting it with a radioactive probe, and then performing autoradiography to reveal probe-complementary DNA. However, host bacteria with plasmid are not plated and allowed to grow without media for days in this experiment.

Which of the Following Is NOT a Part of the Process